@article {Ara{\'U}JO428, author = {Alberto N. Ara{\'U}JO and Jos{\'e} L.F.C. Lima and M. L{\'u}cia M.F.S. Saraiva and Elias A.G. Zagatto}, title = {A New Approach to Dialysis in Sequential Injection Systems: Spectrophotometric Determination of L(+)-Lactate in Wines}, volume = {48}, number = {4}, pages = {428--432}, year = {1997}, doi = {10.5344/ajev.1997.48.4.428}, publisher = {American Journal of Enology and Viticulture}, abstract = {A sequential injection analysis (SIA) system for the spectrophotometric determination of L(+)-lactate using lactate oxidase/peroxidase was developed and applied to wine analysis. It incorporates a dialysis unit between the pump and the selecting valve to provide on-line dilution and interference minimization of the sample matrix. A volume of 100 {\textmu}L of carrier solution trapped in one channel of the dialysis unit, acts as donor and acceptor solution. With an assay cycle of 262 seconds, the system allows a sample throughput of 14 samples per hour and yields precise results (rsd \< 2\%). Peroxidase (0.0512 IU) and 0.0384 IU of lactate oxidase promote the conversion of L(+)-lactate in 160 {\textmu}L of sample, allowing its determination within the 0.25 to 2.5 g/L range. A detection limit of 0.074 g/L was calculated. Results are in good agreement with those obtained with the conventional method (Boehringer UV-Kit).}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/48/4/428}, eprint = {https://www.ajevonline.org/content/48/4/428.full.pdf}, journal = {American Journal of Enology and Viticulture} }