PT - JOURNAL ARTICLE AU - Stéphane Boivin AU - Michel Feuillat AU - Hervié Alexandre AU - Claudine Charpentier TI - Effect of Must Turbidity on Cell Wall Porosity and Macromolecule Excretion of <em>Saccharomyces cerevisiae</em> Cultivated on Grape Juice AID - 10.5344/ajev.1998.49.3.325 DP - 1998 Jan 01 TA - American Journal of Enology and Viticulture PG - 325--332 VI - 49 IP - 3 4099 - http://www.ajevonline.org/content/49/3/325.short 4100 - http://www.ajevonline.org/content/49/3/325.full SO - Am J Enol Vitic.1998 Jan 01; 49 AB - Mannoproteins have a great technological importance such as improvement of proteic and tartaric acid stability or the stimulation of lactic acid bacteria, but their production in wine is still poorly understood. The influence of grape juice turbidity on Saccharomyces cerevisiae cell wall characteristics was studied. Grape juice insoluble particles and colloids were shown to have no influence on yeast cell wall porosity during the lag time but their absence led to increased yeast cell wall porosity at the end of growth phase. Cell wall porosity increased during cell growth and decreased at the transition from the growing phase to the stationary phase. Leakage of cell wall components was linked with cell wall porosity and composition. Such components were freed during growth and during the stationary phase entrance but not during the stationary phase. Compared with a non-clarified medium (380 NTU), a clarified medium (34 NTU) caused the cell wall composition to exhibit a higher proteic content and a lower mannose content. These results suggest that the removal of grape juice particles and colloids has an action on the cell wall synthesis of proliferating yeast i.e. by lowering the glycosylation of mannoproteins. This finding is in agreement with the high cell wall porosity observed and the macromolecule excretion.