TY - JOUR T1 - Identifying Grape Rootstocks With Simple Sequence Repeat (SSR) DNA Markers JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 403 LP - 407 DO - 10.5344/ajev.1998.49.4.403 VL - 49 IS - 4 AU - Hong Lin AU - M. Andrew Walker Y1 - 1998/01/01 UR - http://www.ajevonline.org/content/49/4/403.abstract N2 - Identification of grape rootstocks using morphological features can be difficult because foliar characteristics are variable and often hard to distinguish. This dependence on morphological traits makes rootstock identification nearly impossible during the dormant season, with properly disbudded rootstock plantings, or any time shoots from below the graft union are infrequent. However, cambium tissue from under the rootstock bark provides an alternative sample material for DNA analysis when actively growing shoot tips are not available. DNA was extracted from the leaves and cambium of 58 rootstock selections from the University of California, Davis collections and tested with seven simple sequence repeat (SSR) primers (VVMD5, VVMD6, VVMD7, VVMD28, VVMD31, VVS2, and VVS29). The use of VVMD31, VVS2, VVMD7, and VVMD5 unambiguously identified all of the rootstocks. This technique provides an excellent means of rootstock identification and new variety "fingerprinting". ER -