RT Journal Article
SR Electronic
T1 Development and Characterization of Additional Microsatellite DNA Markers for Grape
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 243
OP 246
DO 10.5344/ajev.1999.50.3.243
VO 50
IS 3
A1 John E. Bowers
A1 Gerald S. Dangl
A1 Carole P. Meredith
YR 1999
UL http://www.ajevonline.org/content/50/3/243.abstract
AB Twenty-two new grape microsatellite markers were developed from CT-repeat regions cloned from Vitis vinifera Pinot noir and Cabernet Sauvignon. These loci were amplified in from 51 to 347 cultivars of V. vinifera. Twelve of the markers are polymorphic and informative in V. vinifera. Five are monomorphic in V. vinifera and the remaining five are polymorphic but less useful because they have only two alleles or more than two bands are amplified per individual. The number of alleles detected in the twelve informative markers ranged from 4 to 19, with heterozygosity values ranging from 0.42 to 0.90. Primer sequences and allele frequencies in the cultivars tested are reported for these markers. Revised allele frequencies are also presented for three previously reported microsatellite markers. Genotypes for the 12 informative new markers are presented for seven suggested reference cultivars.