TY - JOUR T1 - Bioactive Gibberellins in Seeded and Seedless Grapes: Identification and Changes in Content During Berry Development JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 315 LP - 318 DO - 10.5344/ajev.2000.51.4.315 VL - 51 IS - 4 AU - Francisco J. Pérez AU - Camilo Viani AU - Julio Retamales Y1 - 2000/01/01 UR - http://www.ajevonline.org/content/51/4/315.abstract N2 - GA1 and precursors of the early 13-β-hydroxylation pathway of gibberellins biosynthesis GA17 and GA19 were identified in berries of seedless grape cv. Superior seedless by full-scan gas chromatography mass-spectrometry (GC-MS) and Kovats retention indices (KRI). GA1, GA17, GA19, GA3, and its decomposition product gibberellenic acid were also detected in the berries and seeds of Ribier, a seeded cultivar. Biologically active gibberellins were determined throughout fruit development by the α-amylase bioassay. Levels of active gibberellins expressed as equivalent of GA3/g of fresh weight increased at the onset of the first berry growth phase, and at the initiation of the second growth-phase, in berries of both varieties, whereas in seeds maximum levels were attained during the lag phase. GA3 equivalents were 77-fold higher in seeds compared to berries. ER -