TY - JOUR T1 - Interaction of Phylloxera (<em>Daktulosphaira vitifoliae</em> <span class="sc">Fitch</span>) with Grape (<em>Vitis</em> spp.) in Simple Isolation Chambers JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 28 LP - 34 DO - 10.5344/ajev.2001.52.1.28 VL - 52 IS - 1 AU - Astrid Forneck AU - M. Andrew Walker AU - Rolf Blaich AU - Michel Yvon AU - Francois Leclant Y1 - 2001/01/01 UR - http://www.ajevonline.org/content/52/1/28.abstract N2 - A greenhouse-based system to evaluate Daktulosphaira vitifoliae (Fitch) performance on various host plants was modified. Two parthenogenetic generations could be continuously observed and the numbers of reproductive adults and feeding sites (nodosities) determined. Phylloxera performance could be separated into three types. The standard type produced increased numbers of reproducing adults within the first two generations. The second type performed significantly better in the colonizing generation (G1) than the standard type, but had declining reproduction in the second generation (G2). The third type exhibited standard reproduction in G1, but significantly faster and higher reproduction in G2. The isolation chambers were built from 2 L clear plastic water bottles with fine silk-screen tops. These chambers produce quarantine conditions for phylloxera populations, allow continuous monitoring, produce rapid results, and allow inexpensive study of phylloxera performance on selected host plants. Furthermore, the chambers provide the isolation necessary to maintain and rear single-founder phylloxera lines under laboratory conditions.Acknowledgments: We thank Ms. E. J. Frantz for her valuable assistance and Prof. J. Granett (University of California, Davis) for supplying biotype A phylloxera samples. We are grateful for financial assistance provided by the German Research Society (DFG), the California Grape Rootstock Improvement Commission, the California Table Grape Commission, the American Vineyard Foundation, and scholarships provided by the Bundesland Baden-Würtemberg and the German Academic Exchange Service (DAAD). ER -