TY - JOUR T1 - Comparison of Grape Chitinase Activities in Chardonnay and Cabernet Sauvignon with <em>Vitis rotundifolia</em> cv. Fry JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 81 LP - 85 DO - 10.5344/ajev.2005.56.1.81 VL - 56 IS - 1 AU - Steven Van Sluyter AU - Michael J. Durako AU - Christopher J. Halkides Y1 - 2005/03/01 UR - http://www.ajevonline.org/content/56/1/81.abstract N2 - Fungal resistance in Euvitis is generally correlated with levels of pathogenesis-related proteins such as chitinase. Vitis rotundifolia is resistant to many pathogens that affect Vitis vinifera; therefore, grape chitinase activities were compared in V. rotundifolia cv. Fry and V. vinifera cvs. Cabernet Sauvignon and Chardonnay. Cabernet Sauvignon and Chardonnay chitinase activities were approximately 130-fold and 80-fold higher than Fry activities, respectively. Thus, the high pathogen resistance of Fry may be a result of factors other than chitinase. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fry berries had two chitinase isoforms, and at least one was not found in the V. vinifera berries. Cabernet Sauvignon and Chardonnay had four and five chitinase isoforms, respectively. ER -