%0 Journal Article %A Steven Van Sluyter %A Michael J. Durako %A Christopher J. Halkides %T Comparison of Grape Chitinase Activities in Chardonnay and Cabernet Sauvignon with Vitis rotundifolia cv. Fry %D 2005 %R 10.5344/ajev.2005.56.1.81 %J American Journal of Enology and Viticulture %P 81-85 %V 56 %N 1 %X Fungal resistance in Euvitis is generally correlated with levels of pathogenesis-related proteins such as chitinase. Vitis rotundifolia is resistant to many pathogens that affect Vitis vinifera; therefore, grape chitinase activities were compared in V. rotundifolia cv. Fry and V. vinifera cvs. Cabernet Sauvignon and Chardonnay. Cabernet Sauvignon and Chardonnay chitinase activities were approximately 130-fold and 80-fold higher than Fry activities, respectively. Thus, the high pathogen resistance of Fry may be a result of factors other than chitinase. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fry berries had two chitinase isoforms, and at least one was not found in the V. vinifera berries. Cabernet Sauvignon and Chardonnay had four and five chitinase isoforms, respectively. %U https://www.ajevonline.org/content/ajev/56/1/81.full.pdf