PT  - JOURNAL ARTICLE
AU  - Claire-Lise Rosenfield
AU  - Suren Samuelian
AU  - Jose R. Vidal
AU  - Bruce I. Reisch
TI  - Transgenic Disease Resistance in <em>Vitis vinifera</em>: Potential Use and Screening of Antimicrobial Peptides
AID  - 10.5344/ajev.2010.61.3.348
DP  - 2010 Sep 01
TA  - American Journal of Enology and Viticulture
PG  - 348--357
VI  - 61
IP  - 3
4099  - http://www.ajevonline.org/content/61/3/348.short
4100  - http://www.ajevonline.org/content/61/3/348.full
SO  - Am J Enol Vitic.2010 Sep 01; 61
AB  - To increase resistance to important grapevine pathogens, Chardonnay was transformed with a construct containing two antimicrobial peptides (AMPs), Magainin-2 and PGL. Five lines expressing Magainin-2 transcripts showed increased resistance to two strains of Agrobacterium vitis, TM4 and CG450. These results led to a comprehensive study of the effects of nine AMPs on in vitro growth of four pathogens: two bacterial (A. tumefaciens and A. vitis strains CG450 and TM4) and two fungal (Botrytis cinerea and Erysiphe necator). Cecropin B, MSI-99, and Shiva 1 were most effective against the two A. vitis strains, but ESF-12 had no effect. The growth of TM4 was always more sensitive to AMPs than was CG450, a pattern commensurate with the observations of crown gall resistance among transformed vines expressing Magainin-2. Botrytis cinerea required more than 10 μM α-Purothionin, PGL, or ESF39 for effective growth inhibition. None of the AMPs studied could reproducibly control E. necator spore germination or lesion formation. MSI-99 and Cecropin B were most promising for future increased resistance to crown gall disease in transgenic grapevines.