PT - JOURNAL ARTICLE AU - Manuela Pérez-Gilabert AU - Edgar Téllez AU - Francisco García-Carmona TI - Extraction and Partial Purification of β-Galactosidase from Grape Berry Skin (<em>Vitis vinifera</em> L.) with Triton X-114 AID - 10.5344/ajev.2010.61.1.135 DP - 2010 Mar 01 TA - American Journal of Enology and Viticulture PG - 135--139 VI - 61 IP - 1 4099 - http://www.ajevonline.org/content/61/1/135.short 4100 - http://www.ajevonline.org/content/61/1/135.full SO - Am J Enol Vitic.2010 Mar 01; 61 AB - Monastrell grape skins show a high percentage of galactose, likely due to low β-galactosidase activity. Little is known about the characteristics of this enzyme. A simple method to extract and partially purify β-galactosidase from grape berry skin is presented. The kinetic properties of this enzyme are comparable to those of β-galactosidase from other fruits. In this method Triton X-114 (a non-ionic detergent) has a double effect: solubilizing skin components to liberate the enzyme and removing most phenols and hydrophobic proteins, giving rise to a 56-fold increase in specific activity. The method proposed may be useful to easily extract and partially purify this enzyme during grape berry ripening.