PT  - JOURNAL ARTICLE
AU  - Everard J. Edwards
AU  - Alexandra F. Downie
AU  - Peter R. Clingeleffer
TI  - A Simple Microplate Assay to Quantify Nonstructural Carbohydrates of Grapevine Tissues
AID  - 10.5344/ajev.2010.10051
DP  - 2011 Mar 01
TA  - American Journal of Enology and Viticulture
PG  - 133--137
VI  - 62
IP  - 1
4099  - http://www.ajevonline.org/content/62/1/133.short
4100  - http://www.ajevonline.org/content/62/1/133.full
SO  - Am. J. Enol. Vitic.2011 Mar 01; 62
AB  - Knowledge of total nonstructural carbohydrates in plant material is useful for understanding the effects of environmental conditions and vineyard management decisions on grapevine physiology. While many techniques are described in the literature, varying in degree of sophistication, few have been validated in grapevines. We describe the miniaturization of a simple extraction method and microplate based colorimetric assay for the measurement of nonstructural carbohydrates in a range of grapevine tissues. The method provides quantitative results for soluble carbohydrates and starch in samples containing as low as 20 μg carbohydrate.