TY - JOUR T1 - A Simple Microplate Assay to Quantify Nonstructural Carbohydrates of Grapevine Tissues JF - American Journal of Enology and Viticulture JO - Am. J. Enol. Vitic. SP - 133 LP - 137 DO - 10.5344/ajev.2010.10051 VL - 62 IS - 1 AU - Everard J. Edwards AU - Alexandra F. Downie AU - Peter R. Clingeleffer Y1 - 2011/03/01 UR - http://www.ajevonline.org/content/62/1/133.abstract N2 - Knowledge of total nonstructural carbohydrates in plant material is useful for understanding the effects of environmental conditions and vineyard management decisions on grapevine physiology. While many techniques are described in the literature, varying in degree of sophistication, few have been validated in grapevines. We describe the miniaturization of a simple extraction method and microplate based colorimetric assay for the measurement of nonstructural carbohydrates in a range of grapevine tissues. The method provides quantitative results for soluble carbohydrates and starch in samples containing as low as 20 μg carbohydrate. ER -