RT Journal Article
SR Electronic
T1 New Observations on the Integrity, Structure, and Physiology of Flesh Cells from Fully Ripened Grape Berry
JF American Journal of Enology and Viticulture
JO Am. J. Enol. Vitic.
FD American Society for Enology and Viticulture
SP 279
OP 284
DO 10.5344/ajev.2011.10126
VO 62
IS 3
A1 Natacha Fontes
A1 Manuela Côrte-Real
A1 Hernâni Gerós
YR 2011
UL http://www.ajevonline.org/content/62/3/279.abstract
AB The physiological/structural status of the soft ripened berry is still a matter of debate. The isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence, and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function, and viability. Flow cytometry analysis confirmed that protoplasting from grape berry mesocarp tissue yields a single heterogenous population of intact and viable cells. Also, the integrity of the plasma membrane and the architecture and complexity of the intracellular membranous system were shown by FM1-43 staining coupled to confocal microscopy imaging. The observed incorporation of the fluorescent glucose analogue 2-NBDG suggests that endocytosis is involved in the transport and intracellular compartmentation of apoplastic sugars. Neutral Red staining confirmed the intricate organization, size, diversity, and integrity of the vacuolar apparatus that is probably related to the multifaceted roles of the vacuoles in the developing fruit.