@article {Portugalajev.2012.12047, author = {Caur{\'e} Portugal and Fernanda Ruiz-Larrea}, title = {Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines}, elocation-id = {ajev.2012.12047}, year = {2012}, doi = {10.5344/ajev.2012.12047}, publisher = {American Journal of Enology and Viticulture}, abstract = {Brettanomyces/Dekkerais considered as one of the main causes of microbial spoilage and organoleptic deviations of red wines. This work compares sensitiveness and effectiveness of conventional microbiological culture and real-time PCR (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification, and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. It is also concluded that the conventional culture analysis is time-consuming but lower-cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12047}, eprint = {https://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12047.full.pdf}, journal = {American Journal of Enology and Viticulture} }