TY - JOUR T1 - Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of <em>Brettanomyces</em> in Red Wines JF - American Journal of Enology and Viticulture JO - Am. J. Enol. Vitic. DO - 10.5344/ajev.2012.12047 SP - ajev.2012.12047 AU - Cauré Portugal AU - Fernanda Ruiz-Larrea Y1 - 2012/10/22 UR - http://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12047.abstract N2 - Brettanomyces/Dekkerais considered as one of the main causes of microbial spoilage and organoleptic deviations of red wines. This work compares sensitiveness and effectiveness of conventional microbiological culture and real-time PCR (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification, and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. It is also concluded that the conventional culture analysis is time-consuming but lower-cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells. ER -