PT  - JOURNAL ARTICLE
AU  - Chen Li
AU  - Alexandra Erwin
AU  - Dániel Pap
AU  - Courtney Coleman
AU  - Alyssa D. Higgins
AU  - Erzsébet Kiss
AU  - Pál Kozma
AU  - Sarolta Hoffmann
AU  - David W. Ramming
AU  - László G. Kovács
TI  - Selection for <em>Run1-Ren1</em> Dihybrid Grapevines Using Microsatellite Markers
AID  - 10.5344/ajev.2012.12060
DP  - 2012 Oct 22
TA  - American Journal of Enology and Viticulture
PG  - ajev.2012.12060
4099  - http://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12060.short
4100  - http://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12060.full
AB  - A grapevine hybrid progeny was generated to track the inheritance of the Ren1 and the Run1 powdery mildew resistance alleles and the segregation of the powdery mildew resistance phenotype. Genotypic analysis was carried out using flanking microsatellite markers; phenotypic evaluations were done under in vitro and greenhouse conditions. Pairing the phenotypic and genotypic data demonstratedthat Ren1 and Run1 acted as single dominant loci and assorted independentlywithout considerable distortion of segregation. Chromosomal recombination events were detected in the Ren1 but not in the Run1 region, corroborating earlier observations that crossover between homologous chromosomes was suppressed around the Run1 locus. Taken together, the results confirmedthat microsatellite marker-assisted selection is a reliable and expeditious method to combine multiple alleles that confer resistance to a pathogen.