Quantification of the proteins recovered from Incrocio Manzoni wine with different methods by densitometry of SDS-PAGE gels and by Smith assay.
| Protein (μ;g/mL)a | ||
|---|---|---|
| Recovery method | Densitometry | Smith assay |
| aData expressed as the mean ± SD of three independent determinations. | ||
| Dialysis/lyophilization | 27.1 ± 2.0 | 36.4 ± 5.9 |
| KDS method | 19.9 ± 1.8 | 26.0 ± 4.9 |
| Acetone precipitation | 31.8 ± 1.8 | 680.1 ± 111.3 |
| Ethanol precipitation | 31.8 ± 3.1 | 382.8 ± 39.1 |
| (Mean ± SE) | (27.65 ± 2.81) | – |
| TCA precipitation | 9.7 ± 1.2 | 17.4 ± 2.5 |