Summary
Wine yeast strains are characterized by a high chromosomal DNA polymorphism. This can be explained partly by a size difference of different variants of specific chromosomes. This difference can reach up to 45% of the size of the chromosome in question. Two strains, SB1 and Eg8, have a very complex chromosomal pattern and show one band hybridizing with probes from two different chromosomes derived from a reference strain. This is an indication of the presence of “hybrid” chromosomes in these wine strains. The most astonishing result concerns chromosome VIII, frequently present in wine strains in two variant forms. The first normal form has a size of about 580 kb while the second is around 1000 kb. These two forms segregate at meiosis and recombine with a normal chromosome VIII from a laboratory strain. Wine yeasts are thus very different from haploid laboratory strains.
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References
Bakalinsky AT, Snow R (1980) Yeast 6:367–382
Blondin B, Vezinhet F (1988) Rev Fr Oenol 28:7–11
Broach JR, Strathern JN, Hicks JB (1979) Gene 8:121–133
Carle GF, Olson MV (1985) Proc Natl Acad Sci USA. (1985) 82:3756–3760
Carlson M (1986) In: Hicks J (ed) Yeast cell biology. Alan R. Liss Inc., New York, pp 241–250
Carlson M, Botstein D (1982) Cell 28:145–154
Casey GP, Pringle AT, Erdmann PA (1990) J Am Soc Brew Chem 48:100–106
Corcoran LM, Thompson JK, Walliker D, Kemp DJ (1988) Cell 53:807–813
Gallwitz D, Donath C, Sander C (1983) Nature 306:704–707
Gardiner K, Patterson D (1988) Nature 331:371–372
Jager D, Philippsen P (1989) Mol Cell Biol 9:5754–5757
Johnston M, Davis RW (1984) Mol Cell Biol 4:1440–1448
Johnston JR, Mortimer RK (1986) Int J Syst Bacteriol 36:569–572
Lantto RA, Naumova E, Naumov GI, Korhola M (1990) 15th International Conference on Yeast genetics and molecular biology, p 573
Macreadie IG, Jagadish MN, Azad AA, Vaughan PR (1989) Plasmid 21:147–150
Pace T, Ponzi M, Dore E, Janse C, Mons B, Frontali C (1980) Mol Cell Biol 10:6759–6764
Patarapotikul J, Langsley G (1988) Nucleic Acids Res 16:4331–4340
Petering J, Langridge P, Henschke P (1988) Aust NZ Wine Ind J 3:48–52
Rank GH, Casey GP, Xiao W, Pringle AT (1991) Curr Genet 20:189–194
Sheehan CA, Weiss AS (1991) J Inst Brew 97:163–167
Vezinhet F, Blondin B, Hallet JN (1990) Appl Microbiol Biotechnol 32:568–571
Zakian VA, Blanton HM (1988) Mol Cell Biol 8:2257–2260
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Communicated by A. Hinnen
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Bidenne, C., Blondin, B., Dequin, S. et al. Analysis of the chromosomal DNA polymorphism of wine strains of Saccharomyces cerevisiae . Curr Genet 22, 1–7 (1992). https://doi.org/10.1007/BF00351734
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DOI: https://doi.org/10.1007/BF00351734