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Genomic analysis of Grapevine Retrotransposon 1 (Gret1) in Vitis vinifera

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Abstract

The complete sequence of the first retrotransposon isolated in Vitis vinifera, Gret1, was used to design primers that permitted its analysis in the genome of grapevine cultivars. This retroelement was found to be dispersed throughout the genome with sites of repeated insertions. Fluorescent in situ hybridization indicated multiple Gret1 loci distributed throughout euchromatic portions of chromosomes. REMAP and IRAP proved to be useful as molecular markers in grapevine. Both of these techniques showed polymorphisms between cultivars but not between clones of the same cultivar, indicating differences in Gret1 distribution between cultivars. The combined cytological and molecular results suggest that Gret1 may have a role in gene regulation and in explaining the enormous phenotypic variability that exists between cultivars.

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Acknowledgments

We would like to thank Antero Martins and Elsa Gonçalves for providing plant material. We would also like to thank Alexandra Castilho for helpful discussion. H. Sofia Pereira is funded by a postdoctoral fellowship (SFRH/BPD/14549/2003) from Fundação de Ciências e Tecnologia, Portugal.

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Authors and Affiliations

  1. Secção de Genética, Instituto Superior de Agronomia, Centro de Botânica Aplicada à Agricultura, Tapada da Ajuda, 1349-017, Lisboa, Portugal

    H. Sofia Pereira, Augusta Barão, Margarida Delgado, Leonor Morais-Cecílio & Wanda Viegas

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  1. H. Sofia Pereira
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  2. Augusta Barão
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  3. Margarida Delgado
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  4. Leonor Morais-Cecílio
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  5. Wanda Viegas
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Correspondence to H. Sofia Pereira.

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Communicated by J.W. Snape

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Pereira, H.S., Barão, A., Delgado, M. et al. Genomic analysis of Grapevine Retrotransposon 1 (Gret1) in Vitis vinifera . Theor Appl Genet 111, 871–878 (2005). https://doi.org/10.1007/s00122-005-0009-0

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  • DOI: https://doi.org/10.1007/s00122-005-0009-0

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