Skip to main content
Log in

Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Resistance to grapevine powdery mildew is controlled by Run1, a single dominant gene present in the wild grapevine species, Muscadinia rotundifolia, but absent from the cultivated species, Vitis vinifera. Run1 has been introgressed into V. vinifera using a pseudo-backcross strategy, and genetic markers have previously been identified that are linked to the resistance locus. Here we describe the construction of comprehensive genetic and physical maps spanning the resistance locus that will enable future positional cloning of the resistance gene. Physical mapping was performed using a bacterial artificial chromosome (BAC) library constructed using genomic DNA extracted from a resistant V. vinifera individual carrying Run1 within an introgression. BAC contig assembly has enabled 20 new genetic markers to be identified that are closely linked to Run1, and the position of the resistance locus has been refined, locating the gene between the simple sequence repeat (SSR) marker, VMC4f3.1, and the BAC end sequence-derived marker, CB292.294. This region contains two multigene families of resistance gene analogues (RGA). A comparison of physical and genetic mapping data indicates that recombination is severely repressed in the vicinity of Run1, possibly due to divergent sequence contained within the introgressed fragment from M. rotundifolia that carries the Run1 gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aarts MGM, te Lintel Hekkert B, Holub EB, Beynon JL, Stiekema WJ, Pereira A (1998) Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana. Mol Plant Microbe Interact 11:251–258

    CAS  PubMed  Google Scholar 

  • Adam-Blondon A-F, Roux C, Claux D, Butterlin G, Merdinoglu D, This P (2004) Mapping 245 SSR markers on the Vitis vinifera genome: a tool for grape genetics. Theor Appl Genet 109:1017–1027

    Article  CAS  PubMed  Google Scholar 

  • Belkhadir Y, Subramaniam R, Dangl JL (2004) Plant disease resistance protein signaling: NBS-LRR proteins and their partners. Curr Opin Plant Biol 7:391–399

    Article  CAS  PubMed  Google Scholar 

  • Bouquet A (1986) Introduction dans l’espèce Vitis vinifera L. d’un caractère de résistance à l’oidium (uncinula necator Schw. Burr.) issu de l’espèce Muscadinia rotundifolia (Michx.) Small. Vignevini 12[Suppl]:141–146

    Google Scholar 

  • Collins NC, Webb CA, Seah S, Ellis JG, Hulbert SH, Pryor A (1998) The isolation and mapping of disease resistance gene analogues in maize. Mol Plant-Microbe Interact 11:968–978

    CAS  PubMed  Google Scholar 

  • Donald TM, Pellerone F, Adam-Blondon A-F, Bouquet A, Thomas MR, Dry I (2002) Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. Theor Appl Genet 104:610–618

    Article  CAS  PubMed  Google Scholar 

  • Erickson EO, Wilcox WF (1997) Distributions of sensitivities to three sterol demethylation inhibitor fungicides among populations of Uncinula necator sensitive and resistant to triadimefon. Phytopathology 87:784–791

    CAS  Google Scholar 

  • Leister D, Ballvora, AS, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat Genet 14:421–429

    Article  CAS  PubMed  Google Scholar 

  • Lodhi MA, Reisch BI (1995) Nuclear DNA content of Vitis species, cultivars, and other genera of the Viticeae. Theor Appl Genet 90:11–16

    Article  CAS  Google Scholar 

  • Meyers BC, Chin DB, Shen KA, Sivaramakrishnan S, Lavelle DO, Zhang Z, Michelmore RW (1998) The major resistance gene cluster in lettuce is highly duplicated and spans several megabases. Plant Cell 10:1817–1832

    Article  CAS  PubMed  Google Scholar 

  • Milligan SB, Bodeau J, Yaghoobi J, Kaloshian I, Zabel P, Williamson VM (1998) The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes. Plant Cell 10:1307–1319

    Article  CAS  PubMed  Google Scholar 

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  CAS  PubMed  Google Scholar 

  • Olmo HP (1986) The potential role of (Vinifera×Rotundifolia) hybrids in grape variety improvement. Experientia 42:921–926

    Google Scholar 

  • Pauquet J, Bouquet A, This P, Adam-Blondon A-F (2001) Establishment of a local map of AFLP markers around the powdery mildew resistance gene Run1 in grapevine and assessment of their usefulness for marker assisted selection. Theor Appl Genet 103:1201–1210

    Article  CAS  Google Scholar 

  • Pearson RC (1980) Benomyl resistant strains of Uncinula necator on grapes. Plant Dis 64:677–680

    CAS  Google Scholar 

  • Peterson DG, Tomkins JP, Frisch DA, Wing RA, Paterson AH (2000) Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide. J Agric Genomics 5: Published with permission from CAB International. Full text available from http://www.cabi-publishing.org/JAG

    Google Scholar 

  • Riaz S, Dangl GS, Edwards KJ, Meredith CP (2004) A microsatellite marker based framework linkage map of Vitis vinifera L. Theor Appl Genet 108:864–872

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Plainview

    Google Scholar 

  • Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc Natl Acad Sci USA 89:8794–8797

    CAS  PubMed  Google Scholar 

  • Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P (1998) Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068

    Article  CAS  PubMed  Google Scholar 

  • Stirling B, Newcombe G, Vrebalov J, Bosdet I, Bradshaw HD (2001) Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust. Theor Appl Genet 103:1129–1137

    Article  CAS  Google Scholar 

  • Thomas MR, Matsumoto S, Cain P, Scott NS (1993) Repetitive DNA of grapevine—classes present and sequences suitable for cultivar identification. Theor Appl Genet 86:173–180

    CAS  Google Scholar 

  • Thomas MR, Cain P, Scott NS (1994) DNA typing of grapevines—a universal methodology and database for describing cultivars and evaluating genetic relatedness. Plant Mol Biol 25:939–949

    Article  CAS  PubMed  Google Scholar 

  • Vossen EAG van der, van der Voort JNAMR, Kanyuka K, Bendahmane A, Sandbrink H, Baulcombe DC, Bakker J, Stiekema WJ, Klein-Lankhorst RM (2000) Homologues of a single resistance-gene cluster in potato confer resistance to distinct pathogens: a virus and a nematode. Plant J 23:567–576

    Article  CAS  PubMed  Google Scholar 

  • Wei F, Gobel-Werner K, Morroll SM, Kurth J, Mao L, Wing RA, Leister D, Schulze-Lefert P, Wise RP (1999) The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153:1929–1948

    CAS  PubMed  Google Scholar 

  • Woo SS, Jiang JM, Gill BS, Paterson AH, Wing RA (1994) Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucleic Acids Res 22:4922–4931

    CAS  PubMed  Google Scholar 

  • Yang ZN, Mirkov TE (2000) Isolation of large terminal sequences of BAC inserts based on double-restriction-enzyme digestion followed by anchored PCR. Genome 43:412–415

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported in part by the Commonwealth Cooperative Research Centre Program, and specifically by the Cooperative Research Centre for Viticulture (CRCV) and the Grape and Wine Research and Development Corporation (GWRDC). The authors would like to acknowledge the excellent technical assistance of A. Jermakow, K. Swann, N. Tikhomirov and Y. Bertrand.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to C. L. Barker.

Additional information

Communicated by P. Langridge

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barker, C.L., Donald, T., Pauquet, J. et al. Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library. Theor Appl Genet 111, 370–377 (2005). https://doi.org/10.1007/s00122-005-2030-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-2030-8

Keywords

Navigation