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Evaluation of an analytical method for determining phthalate esters in wine samples by solid-phase extraction and gas chromatography coupled with ion-trap mass spectrometer detector

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Abstract

A solid-phase extraction (SPE) method was developed for extraction and analysis of six phthalate esters in wine samples using Carbograph 1 sorbent. The SPE procedure allowed efficient recovery of the investigated phthalates ranging between 78% and 105% with a relative standard deviation (RSD) ≤6.5 for an ethanolic phthalic acid ester (PAE) standard solution and between 73–71% and 96–99% with a RSD ≤8.4 for red wine samples spiked with 20 and 50 ng mL−1 of PAE, respectively. The adsorption isotherms and breakthrough curves for Carbograph 1/water solution were reported. Gas chromatography coupled with an ion-trap mass spectrometer detector (GC/IT-MS) was used for analysis. The instrumental analytical protocol was found to yield a linear calibration in the range 0.01-10.0 μg mL−1 with R 2 values ≥0.9992. The limits of detection in GC/IT-MS (SIM mode) vary between 0.2 and 14 ng mL−1 (RSD ≤5.6) whereas the limits of quantification range between 0.5 and 25 ng mL−1 (RSD ≤5.9); the intra- and inter-day repeatabilities calculated as RSD for wine samples, were between 0.9–7.8 and 1.0–10.5, respectively. The analytical method developed was applied to several commercial wine samples. Furthermore, the investigated methods are simple, reliable, reproducible, and not expensive.

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Authors and Affiliations

  1. Facoltà di Agraria (DISTAAM), Università del Molise, via De Sanctis, 86100, Campobasso, Italy

    Mario Vincenzo Russo, Ivan Notardonato & Giuseppe Cinelli

  2. Chemical Laboratory, DIPIA, INAIL (ex-ISPESL), via Urbana 167, 00184, Rome, Italy

    Pasquale Avino

Authors
  1. Mario Vincenzo Russo
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  2. Ivan Notardonato
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  3. Giuseppe Cinelli
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  4. Pasquale Avino
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Correspondence to Mario Vincenzo Russo.

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Russo, M.V., Notardonato, I., Cinelli, G. et al. Evaluation of an analytical method for determining phthalate esters in wine samples by solid-phase extraction and gas chromatography coupled with ion-trap mass spectrometer detector. Anal Bioanal Chem 402, 1373–1381 (2012). https://doi.org/10.1007/s00216-011-5551-9

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