Skip to main content
SpringerLink
Log in

Construction of a 550 kb BAC contig spanning the genomic region containing the apple scab resistance gene Vf

  • ORIGINAL PAPER
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Abstract

A positional cloning project was started in apple with the aim of isolating the Vf resistance gene of Malus floribunda 821. Vf confers resistance against apple scab, the most important disease in apple orchards. A chromosome walk starting from two molecular markers (M18-CAPS and AM19-SCAR) flanking Vf was performed, using a bacterial artificial chromosome (BAC) library containing inserts of the cultivar Florina, which is heterozygous for Vf. Thirteen BAC clones spanning the region between the two markers were identified in nine chromosome walking steps. The size of the resulting contig is approximately 550 kb. In order to map the Vf region in more detail, we analyzed over 2000 plants from different populations segregating for Vf with markers produced from BAC end sequences. In this way, we were able to restrict the possible location of the Vf gene to a minimum of five clones spanning an interval of approximately 350 kb.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Eidgenössisiche Technische Hochschule Zürich, Bereich Phytomedizin/Pathologie, Universitätsstrasse 2, 8092 Zürich, Switzerland, , , , , , CH

    A. Patocchi, L. Gianfranceschi & C. Gessler

  2. Dipartimento di Colture Arboree, Università di Bologna, 40126 Bologna, Italy, , , , , , IT

    B. A. Vinatzer, S. Tartarini & S. Sansavini

  3. Department of Soil and Crop Sciences, Texas A and M University, College Station, TX 77840, USA, , , , , , US

    H.-B. Zhang

Authors
  1. A. Patocchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. A. Vinatzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Gianfranceschi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Tartarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H.-B. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Sansavini
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. Gessler
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 4 July 1999 / Accepted: 16 September 1999

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patocchi, A., Vinatzer, B., Gianfranceschi, L. et al. Construction of a 550 kb BAC contig spanning the genomic region containing the apple scab resistance gene Vf . Mol Gen Genet 262, 884–891 (1999). https://doi.org/10.1007/s004380051154

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004380051154

Search

Navigation