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Genomics, gene expression and DNA arrays

Nature volume 405, pages 827–836 (2000)Cite this article

Abstract

Experimental genomics in combination with the growing body of sequence information promise to revolutionize the way cells and cellular processes are studied. Information on genomic sequence can be used experimentally with high-density DNA arrays that allow complex mixtures of RNA and DNA to be interrogated in a parallel and quantitative fashion. DNA arrays can be used for many different purposes, most prominently to measure levels of gene expression (messenger RNA abundance) for tens of thousands of genes simultaneously. Measurements of gene expression and other applications of arrays embody much of what is implied by the term ‘genomics’; they are broad in scope, large in scale, and take advantage of all available sequence information for experimental design and data interpretation in pursuit of biological understanding.

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Figure 1: Principal types of arrays used in gene expression monitoring.
Figure 2: Messenger RNA abundance levels in different cells, tissues and organisms.
Figure 3: Methods for analysing gene expression data shown for measurements of expression in the cell cycle of S. cerevisiae.
Figure 4: The ‘guilt-by-association’ method for assigning gene function.
Figure 5: Generic oligonucleotide tag arrays for parallel phenotyping of mutant yeast strains.
Figure 6: Comparative genome hybridization using arrays26,106,107.

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Acknowledgements

We thank S. Fodor, M. Chee, R. Davis, L. Stryer, E. Lander, H. Dong, L. Wodicka, R. Cho, D. Giang, P. Zarrinkar, C. Barlow, J. Gentry, P. Schultz and R. Abagyan for their on-going help and patience, and B. Geierstanger, G. Hampton and S. Kay for helpful comments and a critical reading of the manuscript.

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Lockhart, D., Winzeler, E. Genomics, gene expression and DNA arrays. Nature 405, 827–836 (2000). https://doi.org/10.1038/35015701

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