Optimization of carotenoid production from hyper-producing Rhodotorula glutinis mutant 32 by a factorial approach

Lett Appl Microbiol. 2001 Jul;33(1):12-6. doi: 10.1046/j.1472-765x.2001.00940.x.

Abstract

Aims: Optimization of carotenoid production by a mutant of Rhodotorula glutinis.

Methods: The growth and carotenoid production was optimized in shake flasks using a two-level, three-variable factorial design.

Results: The volumetric carotenoid production could be increased to 129 +/- 2 mg x 1(-1) in a medium containing (g x l(-1)) yeast extract 11.74, glucose 46 and threonine 18 along with other micronutrients, wherein, beta-carotene yield was 102 +/- 2 mg x l(-1), accounting for 80% of the total carotenoids.

Significance and impact of the study: The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of beta-carotene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carotenoids / biosynthesis*
  • Culture Media
  • Glucose / metabolism
  • Mutation
  • Rhodotorula / genetics
  • Rhodotorula / growth & development
  • Rhodotorula / metabolism*
  • Threonine / metabolism
  • beta Carotene / biosynthesis

Substances

  • Culture Media
  • beta Carotene
  • Threonine
  • Carotenoids
  • Glucose