High throughput analysis of red wine and grape phenolics-adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format

J Agric Food Chem. 2007 Jun 13;55(12):4651-7. doi: 10.1021/jf063674n. Epub 2007 May 12.

Abstract

The methyl cellulose precipitable (MCP) tannin assay and a modified version of the Somers and Evans color assay were adapted to high-throughput (HTP) analysis. To improve efficiency of the MCP tannin assay, a miniaturized 1 mL format and a HTP format using 96 well plates were developed. The Somers color assay was modified to allow the standardization of pH and ethanol concentrations of wine samples in a simple one-step dilution with a buffer solution, thus removing inconsistencies between wine matrices prior to analysis and allowing for its adaptation to a HTP format. Validation studies showed that all new formats were efficient, and results were reproducible and analogous to the original formats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catechin / analysis
  • Phenols / analysis*
  • Tannins / analysis*
  • Vitis / chemistry*
  • Wine / analysis*

Substances

  • Phenols
  • Tannins
  • Catechin