Analytical methods for monoterpene glycosides in grape and wine. I. XAD-2 extraction and gas chromatographic-mass spectrometric determination of synthetic glycosides

J Chromatogr. 1992 Jan 31;590(2):313-28. doi: 10.1016/0021-9673(92)85395-a.

Abstract

Synthetic monoterpene and aromatic beta-D-glucopyranosides, beta-rutinosides and 6-O-(alpha-L-arabinofuranosyl)-beta-D-glucopyranosides and their corresponding alcohols, diluted in a synthetic solution imitating wine, were isolated and separated by selective retention on Amberlite XAD-2. The corresponding recoveries and the conditions for the direct determination of these glycosides by gas chromatography and gas chromatography-mass spectrometry after derivatization were determined. Trifluoroacetylation gave the best results but trimethylsilylation provided complementary results. The separation of some diastereoisomeric monoterpene glycosides was also examined.

MeSH terms

  • Fruit / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Glycosides / analysis*
  • Wine / analysis*

Substances

  • Glycosides