… oeni cells submitted or not to heat shock at 42C. Northern blot analysis of the hsp18 gene …
Maximal rate of induction was observed after 5 min of incubation at 42C for clpX and hsp18 …

… One subculture was used as a control and was incubated for 2 h at 37C with no addition,
while the other was supplemented with 3 mM ferulic acid and incubated under the same …

… measurement (pH 7.5, 50C), the two sets ORP … at 50C for 10 min before neutralizing it to a
pH of 6.5 to 7 by KOH during vigorous agitation. After a 10-min centrifugation (6,000 × g at 4C), …

… by their ability to grow at 10 and 45 C, in 6-5 % NaCI, at pH 9·… Enterococcus cecorum failed
to grow at 10 C and in 6-5 % … Grow at 40 0 C but growth at 10 and 45 C is weak. No growth …

… the mixture at 90C for 5 min and at 50C for 10 min. The mixture was placed at 37C, and the
reverse … The mixture was denatured at 80C for 3 min and loaded onto a sequencing gel, and …

… Axelsson, was grown in liquid MRS (14) broth at 37C without shaking, and E. coli was
grown aerobically in Luria-Bertani medium at 37C. For selection and growth of transformants, …

… Colony hybridization was carried out at 60C for 5 h, followed by 5 h at 50C, using standard
procedures as previously described (8). Clones that hybridized with the pdc probe were …

… and incubating for 30 min at 30C. The enzyme purification was performed at 4C. Cells from
2 l of culture were harvested and washed by centrifugation (6000×g, 4C, 15 min) with cold 25 …

… All measurements were performed at 30 C in a 1-mL closed … by incubation for 30 min at 60
C [18]. The binding constant (K i … volume of the cells, C e and C o were the free external probe …

The determination of membrane fluidity in whole cells of Oenococcus oeni was achieved by
membrane probe 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy measurements. …