The structure and function of NAD(H)-glutamate dehydrogenase in plants was studied by
using grapevine (Vitis vinifera L. cv Sultanina) callus grown under different nitrogen sources. …

… cv, Sultanina) grown in the presence of different nitrogen sources (Loulakakis and
Roubelakis-… leaf extract as described previously (Loulakakis and Roubelakis-Angelakis 1990a). …

… GDH activity was determined in the aminating direction by following the change in the A340
(Loulakakis and Roubelakis-Angelakis, 1990a). One unit of GDH activity was defined as the …

… Syntichaki KM, Loulakakis KA, Roubelakis-Angelakis KA: The amino acid sequence similarity
of plant glutamate dehydrogenase with the extremophilic archaeal enzyme conforms to its …

… GDH containing bands were visualised using the tetrazolium system (Loulakakis and
Roubelakis-… KA: The seven NAD(HI_gJutamate dehydrogettase &enzymes exhitit similar anabo!ic …

A modified procedure for efficient isolation of intact and biologically active RNA from leaves,
berries and cell cultures of grapevine (Vitis vinifera L.) is described. RNA is extracted in the …

… In conclusion, the present work confirms our previous report (Loulakakis and Roubelakis-Angelakis
1991) that GDH consists of two subunits, a and 13, which show a correlation with the …

… Grapevine leaf protoplasts were isolated and fractionated as previously described (Loulakakis …
Organellar markers were determined as described earlier (Loulakakis and Roubelakis-…

Nitrogen plays the most important role of all nutrients in plant growth. It is the plant nutrient
most likely to be deficient in grapevine although it is the nutrient most commonly applied to …

Nitrogen (N) plays the most important role of all soil mineral nutrients in plant growth and
development. In grapevine, nitrogen is most likely to be defi cient although it is the main …