New observations on the integrity, structure, and physiology of flesh cells from fully ripened grape berry

N Fontes, M Côrte-Real… - American journal of …, 2011 - Am Soc Enol Viticulture
The physiological/structural status of the soft ripened berry is still a matter of debate. The
isolated mesocarp cells from ripened berries of both wine and table varieties were studied …

Evidence for substantial maintenance of membrane integrity and cell viability in normally developing grape (Vitis vinifera L.) berries throughout development

M Krasnow, M Matthews… - Journal of Experimental …, 2008 - academic.oup.com
Fluorescein diacetate (FDA) was used as a vital stain to assay membrane integrity (cell
viability) in mesocarp tissue of the developing grape (Vitis vinifera L.) berry in order to test …

A method for the isolation of protoplasts from grape berry mesocarp tissue

N Fontes, S Delrot, H Geros - Recent Patents on Biotechnology, 2010 - ingentaconnect.com
As single cell systems, protoplasts have been used in physiological, biochemical and
molecular studies aiming towards the investigation, improvement or modification of plants. In …

Cell death in grape berries: varietal differences linked to xylem pressure and berry weight loss

J Tilbrook, SD Tyerman - Functional Plant Biology, 2008 - CSIRO Publishing
Some varieties of Vitis vinifera L. can undergo berry weight loss during later stages of
ripening. This defines a third phase of development in addition to berry formation and berry …

Confocal measurement of the three‐dimensional size and shape of plant parenchyma cells in a developing fruit tissue

JD Gray, P Kolesik, PB Høj, And… - The Plant …, 1999 - Wiley Online Library
Parenchyma cells from the inner mesocarp of a grape berry (Vitis vinifera L. cv. Chardonnay)
were visualised in three‐dimensions within a whole mount of cleared, stained tissue using …

[HTML][HTML] Purification and functional characterization of protoplasts and intact vacuoles from grape cells

N Fontes, R Silva, C Vignault, F Lecourieux, H Gerós… - BMC research …, 2010 - Springer
Background During grape berry ripening, the vacuoles accumulate water, sugars and
secondary metabolites, causing great impact in plant productivity and wine quality. However …

[HTML][HTML] Proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells

RG Sharathchandra, C Stander, D Jacobson… - PLoS …, 2011 - journals.plos.org
Background This work describes a proteomics profiling method, optimized and applied to
berry cell suspensions to evaluate organ-specific cultures as a platform to study grape berry …

A novel analysis of grapevine berry tissue demonstrates a variety‐dependent correlation between tissue vitality and berry shrivel

S Fuentes, W Sullivan, J Tilbrook… - Australian Journal of …, 2010 - Wiley Online Library
Abstract Background and Aims: For Vitis vinifera L. berries late in ripening, varietal
differences have been found in tissue vitality and water relations linked to berry shrivel …

In situ fixation of grape berries

P Diakou, JP Carde - Protoplasma, 2001 - Springer
Usual immersion protocols in aldehyde solutions fail to fully preserve the fine structure of
both exocarp and mesocarp cells of grape berries, especially for the veraison (onset of …

[HTML][HTML] Morphoanatomical symptomatology and osmotic behavior of grape berry shrivel

B Bondada, M Keller - Journal of the American Society for …, 2012 - journals.ashs.org
Berry shrivel, a physiological disorder, adversely affects ripening of grape (Vitis vinifera L.)
berries; however, its causes are unknown. We adopted a holistic approach to elucidate …