Skip to main content
Advertisement

Main menu

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Print on Demand
  • Information For
    • Authors
    • Open Access Publishing
    • AJEV Preprint and AI Software Policy
    • Submission
    • Subscribers
      • Proprietary Rights Notice for AJEV Online
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN

User menu

  • Log in

Search

  • Advanced search
American Journal of Enology and Viticulture
  • Log in
  • Follow ajev on Twitter
  • Follow ajev on Linkedin
American Journal of Enology and Viticulture

Advanced Search

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Print on Demand
  • Information For
    • Authors
    • Open Access Publishing
    • AJEV Preprint and AI Software Policy
    • Submission
    • Subscribers
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN
Article

Large-Scale Production of Spores to Botrytise Grapes for Commercial Natural Sweet Wine Production

K. E. Nelson, T. Kosuge, Alice Nightingale
Am J Enol Vitic.  1963  14: 118-128  ; DOI: 10.5344/ajev.1963.14.3.118
K. E. Nelson
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
T. Kosuge
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Alice Nightingale
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

A large dependable supply of spores of Botrytis cinerea Pers. was obtained by growing pure cultures of the fungus on sterilized Thompson Seedless grape berries in one-liter Pyrex Roux culture bottles. A 10-ml glass syringe was used to introduce 2-8 ml of a spore suspension through a gauze filter disk covering the bottle opening. The spore suspension was drawn from a 1-2 liter supply through a syphon tube attached to the syringe. One to two-hundred bottles could be inoculated in less than an hour from 2-4 single-spore sporulating test tube cultures. Practically no contamination problems were encountered. A temperature of 20-22°C, a strong source of indirect sunlight and a sterile air supply induced best sporulation. Spores were dry-harvested by a vacuum cyclone separator and could be stored in cotton stoppered flasks at 4°C for at least 10 months without appreciable reduction in viability.

These results make it possible to produce a more economical and dependable supply of spores for inoculating fruit in natural sweet wine production from botrytised grapes. Labor and equipment requirements can be drastically reduced by producing the spores throughout the year and storing them until used.

  • Copyright 1963 by the American Society for Enology and Viticulture

Sign in for ASEV members

ASEV Members, please sign in at ASEV to access the journal online.

Sign in for Institutional and Non-member Subscribers

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 2 day for US$10.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Forgot your user name or password?

PreviousNext
Back to top

Vol 14 Issue 3

  • Table of Contents
  • Index by author
View full PDF
Email Article

Thank you for your interest in spreading the word on AJEV.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Large-Scale Production of Spores to Botrytise Grapes for Commercial Natural Sweet Wine Production
(Your Name) has forwarded a page to you from AJEV
(Your Name) thought you would like to read this article from the American Journal of Enology and Viticulture.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
You have accessRestricted access
Large-Scale Production of Spores to Botrytise Grapes for Commercial Natural Sweet Wine Production
K. E. Nelson, T. Kosuge, Alice Nightingale
Am J Enol Vitic.  1963  14: 118-128  ; DOI: 10.5344/ajev.1963.14.3.118
K. E. Nelson
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. Kosuge
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alice Nightingale
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
You have accessRestricted access
Large-Scale Production of Spores to Botrytise Grapes for Commercial Natural Sweet Wine Production
K. E. Nelson, T. Kosuge, Alice Nightingale
Am J Enol Vitic.  1963  14: 118-128  ; DOI: 10.5344/ajev.1963.14.3.118
K. E. Nelson
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. Kosuge
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alice Nightingale
Department of Plant Pathology, University of California, Davis; Technician, Cresta Blanca Winery, Livermore, California.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
del.icio.us logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More from this TOC section

  • Sparkling Wines Produced from Alternative Varieties: Sensory Attributes and Evolution of Phenolics during Winemaking and Aging
  • Leaf Blade versus Petiole Analysis for Nutritional Diagnosis of Vitis vinifera L. cv. Tempranillo
  • Mechanical Canopy and Crop-Load Management of Pinot gris in a Warm Climate
Show more Article

Similar Articles

AJEV Content

  • Current Volume
  • Archive
  • Best Papers
  • ASEV National Conference Technical Abstracts
  • Print on Demand

Information For

  • Authors
  • Open Access Publishing
  • AJEV Preprint and AI Software Policy
  • Submission
  • Subscribers
  • Permissions and Reproductions

Other

  • Home
  • About Us
  • Feedback
  • Help
  • Alerts
  • ASEV
asev.org

© 2025 American Society for Enology and Viticulture.  ISSN 0002-9254.

Powered by HighWire