Abstract
Callus derived from cultured anthers of Gloryvine (Vitis vinifera L. x V. rupestris Scheele) contained both haploid (n=19) and diploid (2n=38) cells, but all the plantlets produced in vitro were diploid. The presence of haploid cells in callus derived from anthers was further indicated by the occurrence of nuclei with the 1C-DNA level. Haploid cells were not found in callus from internode explants. Multinucleate pollen grains were observed, but there was strong anatomical evidence that the anther-callus originated primarily from somatic cells of the anthers wall and the connective. There was considerable variation among anther-derived plants in leaf morphology. Progeny of selfed anther-derived plants exhibited segregation for leaf characters indicating heterozygosity and somatic origin. Isoenzyme patterns for indophenol oxidase, leucine amino peptidase and catechol oxidase in anther-derived plants and in the original genotype from which the cultures were established were indistinguishable.
- Copyright 1983 by the American Society for Enology and Viticulture
Sign in for ASEV members
ASEV Members, please sign in at ASEV to access the journal online.
Sign in for Institutional and Non-member Subscribers
Log in using your username and password
Pay Per Article - You may access this article (from the computer you are currently using) for 2 day for US$10.00
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
Vol 34 Issue 2
