Abstract
High performance liquid chromatography (HPLC) and diode array spectroscopy (DAS) were applied simultaneously for the separation, identification, and quantification of the anthocyanins of Vitis vinifera variety Tempranillo. Use of diode array detector (DAD) permitted simultaneous recording of the chromatographic analysis at several wavelengths and the visible-ultraviolet (v-uv) spectra of the different anthocyanins separated by HPLC. Using these techniques and on the basis of their elution order and spectroscopic properties, 15 anthocyanins were identified: the 3-monoglucosides of delphinidin, cyanidin, petunidin, peonidin, and malvidin and the derivatives of these five compounds acylated with acetic and p-coumaric acids. Acid and alkaline hydrolysis of both the anthocyanins isolated and the total anthocyanin extract obtained from the grapes confirm the identities of these compounds.
- Received November 1987.
- Copyright 1988 by the American Society for Enology and Viticulture
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Vol 39 Issue 3
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