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Article

Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution

R. M. Marcé, M. Calull, F. Borrull, F. X. Rius
Am J Enol Vitic.  1990  41: 289-294  ; DOI: 10.5344/ajev.1990.41.4.289
R. M. Marcé
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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M. Calull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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F. Borrull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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F. X. Rius
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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Abstract

An optimized method to determine carboxylic acids in wine based on pre-column derivatization with phenacyl bromide is reported. The determination of the different acids has been carried out using a linear gradient elution involving water and methanol. The method has been optimized by the Simplex method using a modification of CRF (chromatographic response factor) as the criterion for selectivity optimization. After validation, the method was applied to determine carboxylic acids in red and white wines and grape must of the Tarragona region.

  • carboxylic acids
  • wine
  • optimization
  • simplex method
  • linear gradient
  • Received November 1989.
  • Copyright 1990 by the American Society for Enology and Viticulture

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You have accessRestricted access
Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution
R. M. Marcé, M. Calull, F. Borrull, F. X. Rius
Am J Enol Vitic.  1990  41: 289-294  ; DOI: 10.5344/ajev.1990.41.4.289
R. M. Marcé
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
  • Find this author on Google Scholar
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M. Calull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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F. Borrull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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F. X. Rius
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
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Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution
R. M. Marcé, M. Calull, F. Borrull, F. X. Rius
Am J Enol Vitic.  1990  41: 289-294  ; DOI: 10.5344/ajev.1990.41.4.289
R. M. Marcé
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Calull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. Borrull
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. X. Rius
Dep. Quimica, Universitat de Barcelona, Imperial Tarraco 1, 43005 Tarragona (Spain).
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
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