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Article

The Use of Isozymes to Identify 60 Grapevine Rootstocks (Vitis spp.)

M. Andrew Walker, Linda Liu
Am J Enol Vitic. January 1995 46: 299-305; published ahead of print January 01, 1995 ; DOI: 10.5344/ajev.1995.46.3.299
M. Andrew Walker
Department of Viticulture and Enology, University of California, Davis, CA 95616-8749.
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Linda Liu
Department of Viticulture and Enology, University of California, Davis, CA 95616-8749.
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Abstract

Starch gel electrophoresis was used to produce isozyme banding patterns for the 60 grape rootstocks available from the University of California, Davis grape collections. Strips of tissue from under the bark consisting of young phloem, young xylem, and vascular cambium provided the sample material for protein extraction. Isozyme patterns were produced with the following enzyme systems: glucose phosphate isomerase (GPI), aspartate aminotransferase (AAT), phosphogluconate dehydrogenase (6-PGD), phosphoglucomutase (PGM), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), leucine amino-peptidase (LAP). The resulting patterns were consolidated and arranged in a grid designed to function as a taxonomic key. A unique isozyme profile was obtained for each rootstock. Ten unknown rootstocks were tested and their identities were determined using this system.

  • ampelography
  • isoenzymes
  • Received September 1994.
  • Copyright 1995 by the American Society for Enology and Viticulture

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The Use of Isozymes to Identify 60 Grapevine Rootstocks (Vitis spp.)
M. Andrew Walker, Linda Liu
Am J Enol Vitic.  January 1995  46: 299-305;  published ahead of print January 01, 1995 ; DOI: 10.5344/ajev.1995.46.3.299

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The Use of Isozymes to Identify 60 Grapevine Rootstocks (Vitis spp.)
M. Andrew Walker, Linda Liu
Am J Enol Vitic.  January 1995  46: 299-305;  published ahead of print January 01, 1995 ; DOI: 10.5344/ajev.1995.46.3.299
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