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Article

Charge Properties of Some Grape and Wine Polysaccharide and Polyphenolic Fractions

A. Vernhet, P. Pellerin, C. Prieur, J. Osmianski, M. Moutounet
Am J Enol Vitic.  1996  47: 25-30  ; DOI: 10.5344/ajev.1996.47.1.25
A. Vernhet
Institut Supérieur de la Vigne et du Vin, Ecole Nationale Supérieure Agronomique de Montpellier - UFR de Technologie-Oenologie, 2 place Pierre Viala, F-34 060 Montpellier Cedex 1
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P. Pellerin
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C. Prieur
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J. Osmianski
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M. Moutounet
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Abstract

Charge densities of the major wine polysaccharides and of grape seed and wine polyphenols have been determined with a method developed by Mütek (Germany), relying on titration with a counter-charged polyelectrolyte associated to streaming potential measurements. Charge densities were determined in a model hydroalcoholic solution at pH 3.5 and in 10 mM KNO3 solutions, from pH 2 to 9. The polysaccharides tested — yeast mannoproteins, grape arabinogalactan-proteins (AGPs), and rhamnogalacturonan RG-II — carried negative charges in the wine pH range. The net charge density of yeast mannoproteins was shown to be related to their phosphorus content and to be pH-independent in the range tested (2 to 9). Absolute charge densities of AGPs and RG-II were related to the dissociation of the carboxylic functions of their uronic acids and increased dramatically in the pH range 2 to 5. Polyphenolic fractions, including native tannins isolated from grape seeds and polyphenolic fractions recovered from wine, carried no charges or negligible charges at pH 3.5. As a consequence, wine polysaccharides may establish electrostatic and ionic interactions, but such interactions will play little, if any, in the association of polyphenols with other components.

  • polysaccharides
  • mannoproteins
  • arabinogalactan
  • rhamnogalacturonan
  • tannins
  • procyanidins
  • charge density
  • interactions
  • Received January 1995.
  • Copyright 1996 by the American Society for Enology and Viticulture
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Charge Properties of Some Grape and Wine Polysaccharide and Polyphenolic Fractions
A. Vernhet, P. Pellerin, C. Prieur, J. Osmianski, M. Moutounet
Am J Enol Vitic.  1996  47: 25-30  ; DOI: 10.5344/ajev.1996.47.1.25
A. Vernhet
Institut Supérieur de la Vigne et du Vin, Ecole Nationale Supérieure Agronomique de Montpellier - UFR de Technologie-Oenologie, 2 place Pierre Viala, F-34 060 Montpellier Cedex 1
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P. Pellerin
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C. Prieur
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J. Osmianski
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M. Moutounet
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Charge Properties of Some Grape and Wine Polysaccharide and Polyphenolic Fractions
A. Vernhet, P. Pellerin, C. Prieur, J. Osmianski, M. Moutounet
Am J Enol Vitic.  1996  47: 25-30  ; DOI: 10.5344/ajev.1996.47.1.25
A. Vernhet
Institut Supérieur de la Vigne et du Vin, Ecole Nationale Supérieure Agronomique de Montpellier - UFR de Technologie-Oenologie, 2 place Pierre Viala, F-34 060 Montpellier Cedex 1
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P. Pellerin
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C. Prieur
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J. Osmianski
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M. Moutounet
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