Abstract
An enzyme-linked immunosorbent assay (ELISA) was compared to the standard woody indexing procedure for effectiveness in detecting grapevine leafroll associated virus (GLRaV) 3 and 4 in infected grapevines of cvs. Pinot noir (PN) and Thompson Seedless (TS). Four plants per plot of individual Cabernet franc (CF) indicator plants were each graft-inoculated with buds from 24 vines of PN and TS infected with the GLRaV 3 and 4, respectively. Inoculated indicators were established in the field, observed periodically for symptoms, and assayed by ELISA. All plots showed GLRaV symptoms and positive ELISA reactions by the end of the first full growing season for GLRaV 3 and by the end of the second full growing season for GLRaV 4. Results on an individual plant basis were similar, whereby 92% to 95% of CF inoculated with GLRaV 3 or GLRaV 4 were positive for symptoms and ELISA. ELISA reactions for GLRaV 3 were consistently higher than those for GLRaV 4. Virus transmission was not dependent on growth of inoculated buds. We conclude that ELISA is useful for rapid detection of GLRaV 3 and 4 in dormant wood of source grapevines and in inoculated CF indicators.
- Copyright 1996 by the American Society for Enology and Viticulture
Sign in for ASEV members
ASEV Members, please sign in at ASEV to access the journal online.
Sign in for Institutional and Non-member Subscribers
Log in using your username and password
Pay Per Article - You may access this article (from the computer you are currently using) for 2 day for US$10.00
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
Vol 47 Issue 3
