Development and Characterization of Additional Microsatellite DNA Markers for Grape

Abstract

Twenty-two new grape microsatellite markers were developed from CT-repeat regions cloned from Vitis vinifera Pinot noir and Cabernet Sauvignon. These loci were amplified in from 51 to 347 cultivars of V. vinifera. Twelve of the markers are polymorphic and informative in V. vinifera. Five are monomorphic in V. vinifera and the remaining five are polymorphic but less useful because they have only two alleles or more than two bands are amplified per individual. The number of alleles detected in the twelve informative markers ranged from 4 to 19, with heterozygosity values ranging from 0.42 to 0.90. Primer sequences and allele frequencies in the cultivars tested are reported for these markers. Revised allele frequencies are also presented for three previously reported microsatellite markers. Genotypes for the 12 informative new markers are presented for seven suggested reference cultivars.