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Article

Use of WL Medium to Profile Native Flora Fermentations

Christina L. Pallmann, James A. Brown, Tammi L. Olineka, Luca Cocolin, David A. Mills, Linda F. Bisson
Am J Enol Vitic. January 2001 52: 198-203; published ahead of print January 01, 2001 ; DOI: 10.5344/ajev.2001.52.3.198
Christina L. Pallmann
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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James A. Brown
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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Tammi L. Olineka
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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Luca Cocolin
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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David A. Mills
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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Linda F. Bisson
Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA USA 95616-8749
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Abstract

Vineyard, winery, barrel, and controlled temperature fermentation samples from a single commercial winery (Luna Vineyards) conducting fermentations with indigenous organisms were plated onto Wallerstein Laboratory Nutrient Agar (WL) to evaluate colony diversity. Seventeen unique colony morphologies were identified. Sequence analysis of the DNA encoding a portion of the large ribosomal 26S rRNA indicated that the colony types defined members of six genera: Hanseniaspora uvarum (Kloeckera apiculata), Saccharomyces cerevisiae, Issatchenkia orientalis, Pichia kluyveri, Candida olephelia, and Metschnikowia. Distinct colony subtypes were identified within the pulcherrimin producers traditionally classified as a single species, Metschnikowia pulcherrima. Sequence analysis of the D1/D2 region of the 26S rDNA of these biotypes showed a high degree of divergence, suggesting that these organisms might define separate species. Analysis of fermentations revealed that colony type as indicated on this medium could be used to monitor the yeast population dynamics.

  • WL media
  • Saccharomyces
  • Kloeckera
  • Metschnikowia
  • native fermentation

Acknowledgments: This research was supported by a grant from the American Vineyard Foundation.

  • Copyright 2001 by the American Society for Enology and Viticulture

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Use of WL Medium to Profile Native Flora Fermentations
Christina L. Pallmann, James A. Brown, Tammi L. Olineka, Luca Cocolin, David A. Mills, Linda F. Bisson
Am J Enol Vitic.  January 2001  52: 198-203;  published ahead of print January 01, 2001 ; DOI: 10.5344/ajev.2001.52.3.198

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Use of WL Medium to Profile Native Flora Fermentations
Christina L. Pallmann, James A. Brown, Tammi L. Olineka, Luca Cocolin, David A. Mills, Linda F. Bisson
Am J Enol Vitic.  January 2001  52: 198-203;  published ahead of print January 01, 2001 ; DOI: 10.5344/ajev.2001.52.3.198
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