Abstract
A high-performance liquid chromatography (HPLC) method requiring no sample preparation was developed that can detect and quantify lysozyme in a wine background and detect potassium sorbate together with lysozyme in the same analysis. Optimal separation of lysozyme from red or white components was achieved with a reverse-phase cation exchange column with a pore size of 1000 nm. Mobile phases were (A) 1% acetonitrile, 0.2% trifluoracetic acid, and 98.8% water and (B) 70% acetonitrile 0.2%, trifluoracetic acid, and 29.8% water with detection at 280 nm. Although HPLC analysis can offer the advantage of detecting active and inactive lysozyme and a greater degree of precision, its detection threshold (5 to 10 mg/L) is at least 10-fold greater than that of the standard enzymatic assay.
- Copyright 2002 by the American Society for Enology and Viticulture
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