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Technical Brief

Comparison of Enzymatic and ISE Methods for Ammonia Measurements in Untreated Red and White Juices and Wines

Seth B. Turbow, Graham H. Wehmeier, David E. Block
Am J Enol Vitic.  2002  53: 158-162  ; DOI: 10.5344/ajev.2002.53.2.158
Seth B. Turbow
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
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Graham H. Wehmeier
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
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David E. Block
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
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Abstract

The use of a standard, commercial enzymatic assay for determination of ammonia in juices and wines, with a focus on red juices and wines, has been investigated. Ammonia standards were prepared in model wine solutions, white juice and wine, and red juice and wine that ranged from 0 to 300 mg/L of added ammonia. Ammonia concentration in all standards was then determined by enzymatic assay and by ion-selective electrode for comparison. Repeatability and linearity of the enzymatic assay were excellent for both red juice and wine with only a simple filtration step as a pretreatment. Replicate measurements had a coefficient of variation of less than 2%, and the correlation coefficients (R) for both juice and wine were greater than 0.99. These results were similar to those found for the ion-selective electrode measurements.

  • Ammonia
  • enzymatic assay
  • ISE
  • red wine
  • juice

Acknowledgment: The authors wish to acknowledge the American Vineyard Foundation for funding this work through the grant, Evaluation of the Influence of Common Viticultural Practices on the Chemical and Sensory Characteristics of Wines.

  • Copyright 2002 by the American Society for Enology and Viticulture

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You have accessRestricted access
Comparison of Enzymatic and ISE Methods for Ammonia Measurements in Untreated Red and White Juices and Wines
Seth B. Turbow, Graham H. Wehmeier, David E. Block
Am J Enol Vitic.  2002  53: 158-162  ; DOI: 10.5344/ajev.2002.53.2.158
Seth B. Turbow
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
  • Find this author on Google Scholar
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Graham H. Wehmeier
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
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David E. Block
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
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Comparison of Enzymatic and ISE Methods for Ammonia Measurements in Untreated Red and White Juices and Wines
Seth B. Turbow, Graham H. Wehmeier, David E. Block
Am J Enol Vitic.  2002  53: 158-162  ; DOI: 10.5344/ajev.2002.53.2.158
Seth B. Turbow
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Graham H. Wehmeier
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David E. Block
Department of Viticulture and Enology, University of California, Davis, One Shields Avenue, Davis, CA 95616.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
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