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Applying Chlorophyll Fluorescence Technique to Cold Hardiness Studies of Grapevines

Hongying Jiang, G. Stanley Howell
Am J Enol Vitic. January 2002 53: 210-217; published ahead of print January 01, 2002
Hongying Jiang
Department of Horticulture, Michigan State University, East Lansing, MI 48824-1325; 1Current address: Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931
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G. Stanley Howell
Department of Horticulture, Michigan State University, East Lansing, MI 48824-1325; 1Current address: Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931
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Abstract

Woody tissue cold hardiness and chlorophyll fluorescence of Concord (Vitis labruscana Bailey) grapevines were measured regularly during the dormant seasons in 1997 to 1998, 1998 to 1999, and late winter 2000. Chlorophyll fluorescence was measured after a controlled freeze-stress. Fv/Fm was used as an indicator of viability. Response magnitude varied with each portion of the dormant season and the type of woody tissues. Fv/Fm temperature inflection points (temperature at which no and 100% tissue injury occurred) were closely related with T50 (temperature at which 50% of the sample was injured) calculated from a regrowth test. Injury thresholds Fv/Fm50 , Fv/Fm50 , and Fv/Fm100 (the Fv/Fm values when no, 50%, and 100% of the tissue were injured, respectively) are proposed for different woody tissues at three periods of the dormant season. Preliminary application of two fluorometers and field measurement was also demonstrated.

  • Concord (Vitis labruscana Bailey)
  • chlorophyll fluorescence
  • Fv/Fm temperature inflection points
  • Fv/Fm injury thresholds
  • viability test

Acknowledgments: The authors gratefully acknowledge the technical assistance of J.A. Flore, F.W. Ewers, B. Rowe, J. Dwyer, S. Breitkreutz, L. Blain, M. C. Vasconcelos, Jun Song, and W. Nail and the statistical assistance of O. Schabenberger.

  • Copyright 2002 by the American Society for Enology and Viticulture

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Applying Chlorophyll Fluorescence Technique to Cold Hardiness Studies of Grapevines
Hongying Jiang, G. Stanley Howell
Am J Enol Vitic.  January 2002  53: 210-217;  published ahead of print January 01, 2002

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Applying Chlorophyll Fluorescence Technique to Cold Hardiness Studies of Grapevines
Hongying Jiang, G. Stanley Howell
Am J Enol Vitic.  January 2002  53: 210-217;  published ahead of print January 01, 2002
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