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Technical BriefTechnical BriefTechnical Briefs

Somatic Embryogenesis from Grapevine Anthers: The Optimal Developmental Stage for Collecting Explants

Ivana Gribaudo, Giorgio Gambino, Rosalina Vallania
Am J Enol Vitic. January 2004 55: 427-430; published ahead of print January 01, 2004
Ivana Gribaudo
Istituto Virologia Vegetale, U.S. Viticoltura, Via Leonardo da Vinci 44, 10095 Grugliasco TO, Italia
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Giorgio Gambino
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Rosalina Vallania
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Abstract

In grapevine, anther culture is often used to obtain somatic embryos. We identified the best developmental stage to initiate immature anther culture, in relation to the microsporogenesis and the outward appearance of anthers and flowers. Three grape genotypes were observed for common features. Six individual stages were identified, from when the pollen mother cells were in premeiotic phase, the inflorescence was compact, and the anthers were translucent green, to the stage when anthers were opaque yellow and uninucleate pollen had been formed. Optimal somatic embryogenesis was obtained for Vitis vinifera L. cvs. Chardonnay and Barbera when explants were collected at early stages, while in the case of 110R, somatic embryogenesis occurred more frequently at stages IV and V.

  • somatic embryogenesis
  • grapevine
  • Vitis
  • microsporogenesis
  • Copyright 2004 by the American Society for Enology and Viticulture

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Vol 55 Issue 4

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Somatic Embryogenesis from Grapevine Anthers: The Optimal Developmental Stage for Collecting Explants
Ivana Gribaudo, Giorgio Gambino, Rosalina Vallania
Am J Enol Vitic.  January 2004  55: 427-430;  published ahead of print January 01, 2004

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Somatic Embryogenesis from Grapevine Anthers: The Optimal Developmental Stage for Collecting Explants
Ivana Gribaudo, Giorgio Gambino, Rosalina Vallania
Am J Enol Vitic.  January 2004  55: 427-430;  published ahead of print January 01, 2004
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