Skip to main content
Advertisement

Main menu

  • Home
  • AJEV Content
    • Current Volume
    • Papers in Press
    • Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Collections
    • Free Sample Issue
  • Information For
    • Authors
    • Open Access and Subscription Publishing
    • Submission
    • Subscribers
      • Proprietary Rights Notice for AJEV Online
    • Permissions and Reproductions
    • Advertisers
  • About Us
  • Feedback
  • Alerts
    • Alerts
    • RSS Feeds
  • Help
  • Login
  • ASEV MEMBER LOGIN

User menu

  • Log in

Search

  • Advanced search
American Journal of Enology and Viticulture
  • Log in
  • Follow ajev on Twitter
  • Follow ajev on Linkedin
American Journal of Enology and Viticulture

Advanced Search

  • Home
  • AJEV Content
    • Current Volume
    • Papers in Press
    • Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Collections
    • Free Sample Issue
  • Information For
    • Authors
    • Open Access and Subscription Publishing
    • Submission
    • Subscribers
    • Permissions and Reproductions
    • Advertisers
  • About Us
  • Feedback
  • Alerts
    • Alerts
    • RSS Feeds
  • Help
  • Login
  • ASEV MEMBER LOGIN
Article

Functional Analyses of the Malolactic Wine Yeast ML01

John I. Husnik, Pascal J. Delaquis, Margaret A. Cliff, Hennie J.J. van Vuuren
Am J Enol Vitic. March 2007 58: 42-52; published ahead of print March 01, 2007 ; DOI: 10.5344/ajev.2007.58.1.42
John I. Husnik
1Ph.D. student, 3Professor and Eagles Chair, Director, Wine Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Suite 231, 2205 East Mall, Vancouver, BC, V6T 1Z4 Canada; 2Research scientists, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Hwy 97 South, Summerland, BC, V0H 1Z0 Canada.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Pascal J. Delaquis
1Ph.D. student, 3Professor and Eagles Chair, Director, Wine Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Suite 231, 2205 East Mall, Vancouver, BC, V6T 1Z4 Canada; 2Research scientists, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Hwy 97 South, Summerland, BC, V0H 1Z0 Canada.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Margaret A. Cliff
1Ph.D. student, 3Professor and Eagles Chair, Director, Wine Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Suite 231, 2205 East Mall, Vancouver, BC, V6T 1Z4 Canada; 2Research scientists, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Hwy 97 South, Summerland, BC, V0H 1Z0 Canada.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Hennie J.J. van Vuuren
1Ph.D. student, 3Professor and Eagles Chair, Director, Wine Research Centre, Faculty of Land and Food Systems, The University of British Columbia, Suite 231, 2205 East Mall, Vancouver, BC, V6T 1Z4 Canada; 2Research scientists, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Hwy 97 South, Summerland, BC, V0H 1Z0 Canada.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
  • For correspondence: hjjvv@interchange.ubc.ca
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Deacidification of grape musts and wines is important for the production of well-balanced wines. The bacterial malolactic fermentation (MLF) process is unreliable and stuck MLF often leads to spoilage of wines and the production of biogenic amines. The genetically engineered wine yeast, ML01, is a Prise de Mousse strain that contains the malate transport gene (mae1) from Schizosaccharomyces pombe and the malolactic gene (mleA) from Oenococcus oeni, stably integrated into the genome at the URA3 locus. Both genes were isolated from wine-related microorganisms and are expressed under control of the Saccharomyces cerevisiae PGK1 promoter and terminator sequences. ML01 is capable of decarboxylating up to 9.2 g/L of malate to equimolar amounts of lactate in Chardonnay grape must during the alcoholic fermentation. ML01 contains no antibiotic resistance marker genes or vector DNA sequences. The presence of the malolactic cassette in the genome does not affect growth, ethanol production, fermentation kinetics, or metabolism of ML01. Wines produced by the ML01 yeast have lower volatile acidity and improved color properties than wines produced with the parental yeast and a bacterial MLF. Analysis of the volatile compounds, sensory analyses, and industrial production of wine indicate that ML01 is suitable for the commercial production of quality wine.

  • wine
  • Oenococcus oeni
  • malolactic fermentation
  • l-malic acid
  • Received June 2006.
  • Revision received August 2006.
  • Copyright © 2007 by the American Society for Enology and Viticulture
View Full Text

Sign in for ASEV members

ASEV Members, please sign in at ASEV to access the journal online.

Sign in for Institutional and Non-member Subscribers

Log in using your username and password

Forgot your user name or password?

Pay Per Article - You may access this article (from the computer you are currently using) for 2 day for US$10.00

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Forgot your user name or password?

PreviousNext
Back to top

Vol 58 Issue 1

  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
View full PDF
Email Article

Thank you for your interest in spreading the word on AJEV.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Functional Analyses of the Malolactic Wine Yeast ML01
(Your Name) has forwarded a page to you from AJEV
(Your Name) thought you would like to read this article from the American Journal of Enology and Viticulture.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
You have accessRestricted access
Functional Analyses of the Malolactic Wine Yeast ML01
John I. Husnik, Pascal J. Delaquis, Margaret A. Cliff, Hennie J.J. van Vuuren
Am J Enol Vitic.  March 2007  58: 42-52;  published ahead of print March 01, 2007 ; DOI: 10.5344/ajev.2007.58.1.42

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
You have accessRestricted access
Functional Analyses of the Malolactic Wine Yeast ML01
John I. Husnik, Pascal J. Delaquis, Margaret A. Cliff, Hennie J.J. van Vuuren
Am J Enol Vitic.  March 2007  58: 42-52;  published ahead of print March 01, 2007 ; DOI: 10.5344/ajev.2007.58.1.42
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Conclusions
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More from this TOC section

  • Sparkling Wines Produced from Alternative Varieties: Sensory Attributes and Evolution of Phenolics during Winemaking and Aging
  • Leaf Blade versus Petiole Analysis for Nutritional Diagnosis of Vitis vinifera L. cv. Tempranillo
  • Mechanical Canopy and Crop-Load Management of Pinot gris in a Warm Climate
Show more Articles

Similar Articles

AJEV Content

  • Current Volume
  • Papers in Press
  • Archive
  • Best Papers
  • ASEV National Conference Technical Abstracts
  • Collections
  • Free Sample Issue

Information For

  • Authors
  • Open Access/Subscription Publishing
  • Submission
  • Subscribers
  • Permissions and Reproductions
  • Advertisers

Alerts

  • Alerts
  • RSS Feeds

Other

  • Home
  • About Us
  • Feedback
  • Help
  • Catalyst
  • ASEV
asev.org

© 2023 American Society for Enology and Viticulture.  ISSN 0002-9254.

Powered by HighWire