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Technical Brief

Effect of Wine Dilution on the Reliability of Tannin Analysis by Protein Precipitation

Jacob Skibsted Jensen, Hans Henrik Malmborg Werge, Max Egebo, Anne S. Meyer
Am J Enol Vitic. March 2008 59: 103-105; published ahead of print February 29, 2008 ; DOI: 10.5344/ajev.2008.59.1.103
Jacob Skibsted Jensen
1Department of Chemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800 Kgs. Lyngby, Denmark, and 2FOSS, Slangerupgade 69, 3400 Hillerød, Denmark.
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  • For correspondence: jsk@foss.dk
Hans Henrik Malmborg Werge
1Department of Chemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800 Kgs. Lyngby, Denmark, and 2FOSS, Slangerupgade 69, 3400 Hillerød, Denmark.
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Max Egebo
1Department of Chemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800 Kgs. Lyngby, Denmark, and 2FOSS, Slangerupgade 69, 3400 Hillerød, Denmark.
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Anne S. Meyer
1Department of Chemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800 Kgs. Lyngby, Denmark, and 2FOSS, Slangerupgade 69, 3400 Hillerød, Denmark.
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Abstract

A reported analytical method for tannin quantification relies on selective precipitation of tannins with bovine serum albumin. The reliability of tannin analysis by protein precipitation on wines having variable tannin levels was evaluated by measuring the tannin concentration of various dilutions of five commercial red wines. Tannin concentrations of both very diluted and concentrated samples were systematically underestimated, which could be explained by a precipitation threshold and insufficient protein for precipitation, respectively. Based on these findings, we have defined a valid range of the tannin response in the protein precipitation-tannin assay, which suffers minimally from these problems.

  • wine
  • tannin analysis
  • protein precipitation
  • Received July 2007.
  • Revision received September 2007.
  • Copyright © 2008 by the American Society for Enology and Viticulture
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Effect of Wine Dilution on the Reliability of Tannin Analysis by Protein Precipitation
Jacob Skibsted Jensen, Hans Henrik Malmborg Werge, Max Egebo, Anne S. Meyer
Am J Enol Vitic.  March 2008  59: 103-105;  published ahead of print February 29, 2008 ; DOI: 10.5344/ajev.2008.59.1.103

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Effect of Wine Dilution on the Reliability of Tannin Analysis by Protein Precipitation
Jacob Skibsted Jensen, Hans Henrik Malmborg Werge, Max Egebo, Anne S. Meyer
Am J Enol Vitic.  March 2008  59: 103-105;  published ahead of print February 29, 2008 ; DOI: 10.5344/ajev.2008.59.1.103
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