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Article

Transgenic Disease Resistance in Vitis vinifera: Potential Use and Screening of Antimicrobial Peptides

Claire-Lise Rosenfield, Suren Samuelian, Jose R. Vidal, Bruce I. Reisch
Am J Enol Vitic.  2010 61: 348-357 ; DOI: 10.5344/ajev.2010.61.3.348
Claire-Lise Rosenfield
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Suren Samuelian
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Jose R. Vidal
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Bruce I. Reisch
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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  • For correspondence: bruce.reisch@cornell.edu
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Abstract

To increase resistance to important grapevine pathogens, Chardonnay was transformed with a construct containing two antimicrobial peptides (AMPs), Magainin-2 and PGL. Five lines expressing Magainin-2 transcripts showed increased resistance to two strains of Agrobacterium vitis, TM4 and CG450. These results led to a comprehensive study of the effects of nine AMPs on in vitro growth of four pathogens: two bacterial (A. tumefaciens and A. vitis strains CG450 and TM4) and two fungal (Botrytis cinerea and Erysiphe necator). Cecropin B, MSI-99, and Shiva 1 were most effective against the two A. vitis strains, but ESF-12 had no effect. The growth of TM4 was always more sensitive to AMPs than was CG450, a pattern commensurate with the observations of crown gall resistance among transformed vines expressing Magainin-2. Botrytis cinerea required more than 10 μM α-Purothionin, PGL, or ESF39 for effective growth inhibition. None of the AMPs studied could reproducibly control E. necator spore germination or lesion formation. MSI-99 and Cecropin B were most promising for future increased resistance to crown gall disease in transgenic grapevines.

  • antimicrobial peptide
  • Vitis vinifera
  • Agrobacterium vitis
  • Botrytis cinerea
  • genetic transformation
  • Erysiphe necator
  • Received July 2009.
  • Revision received February 2010.
  • Accepted April 2010.
  • Published online September 2010
  • Copyright © 2010 by the American Society for Enology and Viticulture
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Transgenic Disease Resistance in Vitis vinifera: Potential Use and Screening of Antimicrobial Peptides
Claire-Lise Rosenfield, Suren Samuelian, Jose R. Vidal, Bruce I. Reisch
Am J Enol Vitic.  2010  61: 348-357  ; DOI: 10.5344/ajev.2010.61.3.348
Claire-Lise Rosenfield
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Suren Samuelian
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Jose R. Vidal
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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Bruce I. Reisch
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
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  • For correspondence: bruce.reisch@cornell.edu

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Transgenic Disease Resistance in Vitis vinifera: Potential Use and Screening of Antimicrobial Peptides
Claire-Lise Rosenfield, Suren Samuelian, Jose R. Vidal, Bruce I. Reisch
Am J Enol Vitic.  2010  61: 348-357  ; DOI: 10.5344/ajev.2010.61.3.348
Claire-Lise Rosenfield
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Suren Samuelian
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
  • Find this author on Google Scholar
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Jose R. Vidal
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
  • Find this author on Google Scholar
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  • Search for this author on this site
Bruce I. Reisch
1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: bruce.reisch@cornell.edu
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