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Article

Prevalence and Biodiversity of Brettanomyces bruxellensis in Wine from Northwestern Italy

Simona Campolongo, Kalliopi Rantsiou, Manuela Giordano, Vincenzo Gerbi, Luca Cocolin
Am J Enol Vitic. December 2010 61: 486-491; published ahead of print December 01, 2010 ; DOI: 10.5344/ajev.2010.10034
Simona Campolongo
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Kalliopi Rantsiou
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Manuela Giordano
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Vincenzo Gerbi
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Luca Cocolin
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Abstract

Brettanomyces bruxellensis is a catalyst in the transformation of hydroxycinnamic acids, compounds that naturally occur in grapes, producing volatile phenols that reduce the sensory quality of wine. Depending on their concentrations, volatile phenols can confer off-odors described as phenolic, animal, mousy, wet wool, medicinal, smoky, and spicy. We used a multidisciplinary approach for the detection and quantification of the presence of B. bruxellensis in 87 Italian wines, applying culture-independent and dependent methods (quantitative PCR and traditional microbiological analysis, respectively). Headspace solid-phase microextraction was used to quantify ethylphenols and vinylphenols. Results showed that there was no correlation between culture-dependent and culture-independent methods. Strain biodiversity was investigated by SAU-PCR and showed a differentiation of the isolates based on geographical origin.

  • Brettanomyces bruxellensis
  • quantitative PCR
  • ethylphenols
  • biodiversity
  • SAU-PCR
  • Copyright © 2010 by the American Society for Enology and Viticulture
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Prevalence and Biodiversity of Brettanomyces bruxellensis in Wine from Northwestern Italy
Simona Campolongo, Kalliopi Rantsiou, Manuela Giordano, Vincenzo Gerbi, Luca Cocolin
Am J Enol Vitic.  December 2010  61: 486-491;  published ahead of print December 01, 2010 ; DOI: 10.5344/ajev.2010.10034

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Prevalence and Biodiversity of Brettanomyces bruxellensis in Wine from Northwestern Italy
Simona Campolongo, Kalliopi Rantsiou, Manuela Giordano, Vincenzo Gerbi, Luca Cocolin
Am J Enol Vitic.  December 2010  61: 486-491;  published ahead of print December 01, 2010 ; DOI: 10.5344/ajev.2010.10034
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