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Tecnical Brief

An Enhanced Method for Vitis vinifera L. DNA Extraction from Wines

Leonor Pereira, Henrique Guedes-Pinto, Paula Martins-Lopes
Am J Enol Vitic.  2011  62: 547-552  ; DOI: 10.5344/ajev.2011.10022
Leonor Pereira
1PhD Student, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
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  • For correspondence: leopereira@utad.pt
Henrique Guedes-Pinto
2Director, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
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Paula Martins-Lopes
3Assistant Professor, School of Life Sciences and Environment, Department of Genetics and Biotechnology, and Researcher, IBB-CGB/UTAD, P.O. Box 1013, 5000-911 Vila Real, Portugal
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Abstract

Wine quality and value largely depend on grape variety, which is of primary importance in wine identification. The aim of the present work was to enhance a wine DNA extraction protocol and, subsequently, grapevine variety identification. This enhanced method is an outcome from several previously developed extraction methods and effectively allows obtaining large amounts of high-quality DNA exhibiting an optimal 260/280 ratio. Grapevine variety DNA extracted from wine was amplifiable with a specific SSR primer. This procedure was applicable for monovarietal and older commercial red and white wines. The potential of this enhanced method relies on its use for traceability as part of protecting both consumer and producer interests.

  • wine
  • DNA extraction
  • grapevine identification
  • SSR marker
  • traceability
  • © 2011 by the American Society for Enology and Viticulture
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An Enhanced Method for Vitis vinifera L. DNA Extraction from Wines
Leonor Pereira, Henrique Guedes-Pinto, Paula Martins-Lopes
Am J Enol Vitic.  2011  62: 547-552  ; DOI: 10.5344/ajev.2011.10022
Leonor Pereira
1PhD Student, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
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  • For correspondence: leopereira@utad.pt
Henrique Guedes-Pinto
2Director, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
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Paula Martins-Lopes
3Assistant Professor, School of Life Sciences and Environment, Department of Genetics and Biotechnology, and Researcher, IBB-CGB/UTAD, P.O. Box 1013, 5000-911 Vila Real, Portugal
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An Enhanced Method for Vitis vinifera L. DNA Extraction from Wines
Leonor Pereira, Henrique Guedes-Pinto, Paula Martins-Lopes
Am J Enol Vitic.  2011  62: 547-552  ; DOI: 10.5344/ajev.2011.10022
Leonor Pereira
1PhD Student, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
  • Find this author on Google Scholar
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  • For correspondence: leopereira@utad.pt
Henrique Guedes-Pinto
2Director, Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB-CGB/UTAD), P.O. Box 1013, 5000-911 Vila Real, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paula Martins-Lopes
3Assistant Professor, School of Life Sciences and Environment, Department of Genetics and Biotechnology, and Researcher, IBB-CGB/UTAD, P.O. Box 1013, 5000-911 Vila Real, Portugal
  • Find this author on Google Scholar
  • Find this author on PubMed
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