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Research Note

Mycobiota of Barbera Grapes from the Piedmont Region from a Single Vintage Year

Valentina Alessandria, Fabio Marengo, Vasileios Englezos, Vincenzo Gerbi, Kalliopi Rantsiou, Luca Cocolin
Am J Enol Vitic.  2015  66: 244-250  ; DOI: 10.5344/ajev.2014.14071
Valentina Alessandria
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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  • For correspondence: valentina.alessandria@unito.it
Fabio Marengo
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vasileios Englezos
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vincenzo Gerbi
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Kalliopi Rantsiou
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Luca Cocolin
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Abstract

In this study, a description of the fungal populations present on grapes of the Barbera cultivar (Vitis vinifera L.) is reported. Grapes from 15 vineyards in Montferrat, an area located in the Piedmont region in northwest Italy, were sampled at maturation during vintage 2012. The grapes were analyzed by culture-dependent and independent methods. Using plate counts on Wallerstein Laboratory Nutrient agar, we determined a yeast load of about 100 colony forming units (cfu)/mL on the grapes. In total, 206 fungal isolates were identified by two different molecular approaches. Highly reproducible and comparable results were obtained with two identification techniques, that is, PCR-based RFLP analysis of the 5.8S internal transcribed spacer (ITS) rRNA region and PCR–DGGE coupled with sequencing of the 26S rRNA gene, confirming that these two methods reliably identify wine yeasts. Both techniques identified a mycobiota mainly composed of Aureobasidium pullulans (73% of the isolates), followed by Rhodotorula glutinis (12%), Hanseniaspora spp. (8%), Issatchenkia terricola (5%), Torulaspora delbrueckii (1%), and Cryptococcus carnescens (1%). Moreover, PCR–DGGE based on DNA and RNA extracted directly from grapes confirmed the presence of A. pullulans, H. uvarum, and Starmerella bacillaris (synonym Candida zemplinina).

  • grape mycobiota
  • ITS sequencing
  • DGGE
  • culture-independent methods
  • Received July 2014.
  • Revision received September 2014.
  • Accepted November 2014.
  • Published online May 2015
  • ©2015 by the American Society for Enology and Viticulture
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Mycobiota of Barbera Grapes from the Piedmont Region from a Single Vintage Year
Valentina Alessandria, Fabio Marengo, Vasileios Englezos, Vincenzo Gerbi, Kalliopi Rantsiou, Luca Cocolin
Am J Enol Vitic.  2015  66: 244-250  ; DOI: 10.5344/ajev.2014.14071
Valentina Alessandria
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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  • For correspondence: valentina.alessandria@unito.it
Fabio Marengo
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vasileios Englezos
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vincenzo Gerbi
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Kalliopi Rantsiou
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Luca Cocolin
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Mycobiota of Barbera Grapes from the Piedmont Region from a Single Vintage Year
Valentina Alessandria, Fabio Marengo, Vasileios Englezos, Vincenzo Gerbi, Kalliopi Rantsiou, Luca Cocolin
Am J Enol Vitic.  2015  66: 244-250  ; DOI: 10.5344/ajev.2014.14071
Valentina Alessandria
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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  • For correspondence: valentina.alessandria@unito.it
Fabio Marengo
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vasileios Englezos
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Vincenzo Gerbi
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Kalliopi Rantsiou
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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Luca Cocolin
1University of Torino, DISAFA, Largo Paolo Braccini 2, 10095 Grugliasco, Italy
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