Influence of Oenococcus oeni and Brettanomyces bruxellensis on Wine Microbial Taxonomic and Functional Potential Profiles

Supplemental Data

Supplemental Table 1  Reads abundance statistics and number of microbial species identifications.

Supplemental Table 2  Differentially abundant pathways with putative relation to microbial survival/interactions in enological conditions with different inoculations of Oenococcus oeni and Brettanomyces bruxellensis strains.

Supplemental Table 3  Top 10 abundant nt-database microbial identifications of the unmapped reads from sample Control_1, top 10 abundant nt-database microbial identifications of the unmapped reads from sample Control_2, and top 10 abundant nt-database microbial identifications of the unmapped reads from sample OEN_23.

Supplemental Figure 1  Principal component analysis (PCA) of the functional potential profile of all samples. Brettanomyces bruxellensis strains CBS 73 (BA), CBS 2336 (BB), and CBS 2499 (BC). Oenococcus oeni strains esterase positive (OEP) and esterase negative (OEN). PCA of the counts of all filtered assembled genes from all samples, including those with lowest (OEN_23) and deepest (OEN-BB_18) sequencing.

Supplemental Figure 2  Plasmid and fungal principal component analysis (PCA). Brettanomyces bruxellensis strains CBS 73 (BA), CBS 2336 (BB), and CBS 2499 (BC). Oenococcus oeni strains esterase positive (OEP) and esterase negative (OEN). (A) Plasmid and (B) fungal taxonomic PCA.

Supplemental Figure 3  Evaluation of the effect of sequencing depth on functional potential profiling. Brettanomyces bruxellensis strains CBS 73 (BA), CBS 2336 (BB), and CBS 2499 (BC). Oenococcus oeni strains esterase positive (OEP) and esterase negative (OEN). (A) Comparison of the number of assembled contigs, identified genes, and total nonredundant (nr) genes per sample. (B) Total nr genes per sample compared to the total number of cleaned reads (scale is in thousands).

Files in this Data Supplement:

• Supplemental Data