Abstract
Polyphenol oxidase (PPO) inhibitors were isolated from grape skin and identified as quercetin derivatives. Among the inhibitors isolated, quercetin-3-glucoside (Q3Glc) was the most potent. Kinetic analysis of the inhibitory activity toward PPO was carried out with authentic Q3Glc using catechol as substrate. The inhibition was reversible, the inhibitory mechanism obtained from Lineweaver-Burk plots indicated that Q3Glc is a competitive inhibitor, and the inhibitory constant (Ki) was 84.6 µM.
- Received December 2017.
- Revision received June 2018.
- Accepted June 2018.
- Published online January 2019
- ©2019 by the American Society for Enology and Viticulture
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Vol 70 Issue 1
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